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Ready for use IVD FISH probe kits

Ready For use IVD FISH Probe Kits 

By Fluorescence In Situ Hybridization (FISH) Method


Non-Small Cell Lung Cancer

Cat#

Product Name

Probe Abbr.

Kit Composition & Size

FP-002

Human ALK gene fusion detection probe

ALK (CE-IVD)


- Fast probe100µL (10T), 

- Pretreatment reagent kit (20T),

- DAPI Staining solution (20T),

- Washing solution (20T).


FP-006


ROS1 (6q22) gene break apart rearrangement detection probe


ROS1 (CE-IVD)

FP-046

C-MET gene detection probe

C-MET/CEP7 (CE-IVD)

 

Breast Cancer

Cat#

Product Name

Probe Abbr.

Kit Composition & Size

FP-001

Human HER-2 gene amplification detection probe

HER2/CEP17 (CE-VD)

- Pretreatment reagent kit (20T),

- Fast probe100µL (10T),

- DAPI Staining solution (20T),

- Washing solution (20T).

FP-008

TOP2A gene amplification probe detection kit

TOP2A (CE-IVD)

 Stomach Cancer

Cat#

Product Name

Probe Abbr.

Kit Composition & Size


FP-001


Human HER-2 gene amplification detection probe


HER2/CEP17 (CE-IVD)

- Pretreatment reagent kit (20T),

- Fast probe100µL (10T),

- DAPI Staining solution (20T),

- Washing solution (20T).

Bladder Cancer

Cat#

Product Name

Probe Abbr.

Kit Composition & Size

FP-009-1


Bladder Cancer cells chromosome and gene anomaly detection probe

CEP3/CEP7 (CE-IVD)

- Pretreatment reagent kit (20T),

- Fast probe100µL (10T),

- DAPI Staining solution (20T),

- Washing solution (20T).

FP-009-2

P16/CEP17 (CE-IVD)

FP-014-2

P53 gene probe

P53/CEP17 (CE-IVD)

Cervical Cancer

Cat#

Product Name

Probe Abbr.

Kit Composition & Size


FP-013


TERC gene amplification detection probe


TERC (CE-IVD)

- Pretreatment reagent kit (20T),

- Fast probe100µL (10T),

- DAPI Staining solution (20T),

- Washing solution (20T).

Brain Cancer

Cat#

Product Name

Probe Abbr.

    Kit Composition & Size

FP-045

1p/19q gene deletion detection probe

1p/19q (CE-IVD)

- Pretreatment reagent kit (20T),

- Fast probe100µL (10T),

- DAPI Staining solution (20T),

- Washing solution (20T).

FP-016

BRAF gene break apart rearrangement detection probe

BRAF (CE-IVD)

Lymphoma

Cat#

Product Name

Probe Abbr.

Kit Composition & Size

FP-024

CCND1 (BCL1)/IGH gene fusion probe

CCND1/IGH (CE-IVD)






- Pretreatment reagent kit (20T),

- Fast probe100µL (10T),

- DAPI Staining solution (20T),

- Washing solution (20T).

FP-014-2

P53 gene probe

P53/CEP17 (CE-IVD)

FP-040

BCL2/IGH t (14;18) gene fusion probe

IGH/BCL2 (CE-IVD)

FP-039

MYC/IGH t (8;14) gene fusion probe

IGH/C-MYC (CE-IVD)

FP-038

BCL6 gene break apart rearrangement probe

BCL6 (CE-IVD)

FP-037

BCL2 gene break apart rearrangement probe

BCL2 (CE-IVD)

FP-033

MYC gene break apart rearrangement probe

MYC (CE-IVD)

FP-023

IGH gene break apart rearrangement probe

IGH (CE-IVD)

Chronic Lymphocytic Leukemia (CLL)

Cat#

Product Name

Probe Abbr.

Kit Composition & Size

FP-014-1


CLL chromosome and gene anomaly detection probe

RB1/ATM (CE-IVD)





- Pretreatment reagent kit (20T),

- Fast probe100µL (10T),

- DAPI Staining solution (20T),

- Washing solution (20T).

FP-014-2

P53/CEP17 (CE-IVD)

FP-014-3

D13S319/CEP12 (CE-IVD)

FP-025

13(13q14)/13q34 probe

D13S319/LAMP1 (13q34) 

(CE-IVD)

FP-034

Chromosome 12 centromeric probe

CEP12 (CE-IVD)

FP-035

ATM gene deletion probe

CEP11/ATM (CE-IVD)

FP-036

MYB (6q23) gene probe

MYB/CEP6 (CE-IVD)

Acute Myeloid Leukemia (AML)

Cat#

Product Name

Probe Abbr.

Kit Composition & Size

FP-005

PML/RARA gene fusion probe

PML/RARA (CE-IVD)



- Pretreatment reagent kit (20T),

- Fast probe100µL (10T),

- DAPI Staining solution (20T),

- Washing solution (20T).

FP-026

KMT2A (MLL) gene break apart rearrangement detection probe

MLL (CE-IVD)

FP-027

CBFB gene break apart rearrangement detection probe

CBFB

FP-028

CBFB/MYH11 gene fusion probe

CBFB/MYH11

FP-044

RUNX1/RUNX1T1 t (8;21) gene fusion probe

AML1/ETO (CE-IVD)

 Chronic Myeloid Leukemia (CML)

Cat#

Product Name

Probe Abbr.

Kit Composition & Size

FP-003

BCR/ABL gene fusion detection probe

BCR/ABL (CE-IVD)

- Pretreatment reagent kit (20T),

- Fast probe100µL (10T),

- DAPI Staining solution (20T),

- Washing solution (20T).

FP-168

ASS gene deletion probe detection kit

ASS (CE-IVD)

Acute Lymphoblastic Leukemia (ALL)

Cat#

Product Name

Probe Abbr.

Kit Composition & Size

FP-003

BCR/ABL gene fusion detection probe

BCR/ABL (CE-IVD)









- Pretreatment reagent kit (20T),

- Fast probe100µL (10T),

- DAPI Staining solution (20T),

- Washing solution (20T).

FP-023

IGH gene break apart rearrangement probe

IGH (CE-IVD)

FP-026

KMT2A (MLL) gene break apart rearrangement detection probe

MLL (CE-IVD)

FP-029

ETV6 (TEL)/RUNX1 (AML1) gene translocation probe

TEL/AML1 (CE-IVD)

FP-030

Chromosomes 4, 10 probe

CEP4/CEP10 (CE-IVD)

FP-031

Chromosome 17 centromeric probe

CEP17 (CE-IVD)

FP-032

P16 gene deletion probe (Tissue)

P16 gene deletion probe (Cell)

P16 (CDK2NA)/CEP9 

(CE-IVD)

FP-033

MYC gene break apart rearrangement probe

MYC (CE-IVD)

FP-062

TCF3/PBX1gene fusion probe

TCF3/PBX1 (CE-IVD)

FP-183

E2A gene break apart probe

E2A (CE-IVD)

FP-186

CRLF2(Xp22/Yp11) gene probe

CRLF2 (CE-IVD)

Multiple Myeloma (MM)

Cat#

Product Name

Probe Abbr.

Kit Composition & Size

FP-014-2

P53 gene probe

P53/CEP17 (CE-IVD)



- Pretreatment reagent kit (20T),

- Fast probe100µL (10T),

- DAPI Staining solution (20T),

- Washing solution (20T).

FP-021

RB1 gene deletion probe

RB1 (CE-IVD)

FP-023

IGH gene break apart probe

IGH (CE-IVD)

FP-024

CCND1 (BCL1)/IGH gene fusion probe

CCND1/IGH (CE-IVD)

FP-025

13(13q14)/13q34 probe

D13S319/LAMP1 (13q34) 

(CE-IVD)

FP-206-1

1q21 gene probe

1q21 (CE-IVD)

FP-206-2

1p36/1q25 gene probe

1p36/1q25 (CE-IVD)

Myelodysplastic Syndrome (MDS)

Cat#

Product Name

Probe Abbr.

Kit Composition & Size

FP-011-1




MDS chromosome and gene anomaly detection probe

D7S486/CEP7 (CE-IVD)




- Pretreatment reagent kit (20T),

- Fast probe100µL (10T),

- DAPI Staining solution (20T),

- Washing solution (20T).

FP-011-2

D7S522/CEP7 (CE-IVD)

FP-011-3

CSF1R/D5S630 (CE-IVD)


FP-011-4

EGR1/D5S630 5P15(D5S630)/5q31.2 

(CE-IVD)

FP-011-5

D20S108/CEP8 (CE-IVD)

FP-011-6

CEPX/CEPY (CE-IVD)

FP-018

Chromosome 8 centromeric probe

CEP8 (CE-IVD)

Aplastic Anemia

Cat#

Product Name

Probe Abbr.

Kit Composition & Size

FP-011-1

7q chromosome deletion [del(7q)] probe

D7S486/CEP7 (CE-IVD)



- Pretreatment reagent kit (20T),

- Fast probe100µL (10T),

- DAPI Staining solution (20T),

- Washing solution (20T).


FP-011-4


5q chromosome deletion [del(5q)] probe

EGR1/D5S630 5P15(D5S630)/5q31.2 

(CE-IVD)

FP-020

20q chromosome deletion [del(20q)] probe

20q12(D20S108

20q13.12 (CE-IVD)

FP-018

Chromosome 8 centromeric probe

CEP8 (CE-IVD)

Soft Tissue Cancer

Cat#

Product Name

Probe Abbr.

Kit Composition & Size


FP-054


MDM2 gene amplification probe


MDM2 (CE-IVD)

- Pretreatment reagent kit (20T),

- Fast probe100µL (10T),

- DAPI Staining solution (20T),

- Washing solution (20T).

FP-055

SS18(SYT) gene break apart probe

SS18(SYT) (CE-IVD)


Flexible Dye Selection

Fluorescein color

Excitation wavelength

Emission wavelength

Filter

Orange (RHO)

553

565

Orange Monochannel ; Green/Orange Dual Channel

Green (FITC)

495

518

Green Monochannel ; Green/Orange Dual Channel

DAPI (Blue)

367

452

Monochannel


Our Advantages:

a). FISH probes (FastProbe®) hybridization time of 2 hours (blood cell and tissue) against 16-24 hours for conventional probes in the market. 

b). FISH probes (FastProbe®) using the Non-Repetitive Sequences technology ensuing a product with a very good hybridization result, good and clear signal to background noise, high specificity and accuracy.

c). FISH probes (FastProbe®) with a very good anti-quenching effect allowing long observation under fluorescence microscope.

d). An excellent customer service with very short lead time from PO confirmation to shipment.

e). Very competitive affordable price. 


Fast Fluorescence In-Situ Hybridization (FISH) Probe

Protocol Manual

        1. SAMPLE

        A. Tissue

        (1) a). Paraffin-embedded tissue samples fixation with 4% formaldehyde for 6-72 hours.

           b). Thickness: 3-5µm

        (2) Glass slides: Adhesive slides.

         

        B. Cells

        (1) a). Anticoagulated fresh bone marrow (Storage conditions 4oC, within 24h).

           b). Bone marrow cells accurate fixation (Storage conditions -20oC, within 6 months).

        (2) Glass slides: Ordinary/Common glass slides.

         

        2. SAMPLE PRETREATMENT 

        Pretreatment procedure

        A. Tissue - Slides pretreatment (sample releasing agent)

        1. Slides heating: Heat at 80oC for 30min or 65oC for 2h or overnight.

        2. Dewaxing: Deparaffinization agent 68oC for 15min.

        3. Washing: Wash with anhydrous EtOH at room temperature for 5min.

        4. Permeation: Permeation agent 90oC for 20min or deionized water 90oC for 40min.

        5. Washing: Wash with distilled water at 37oC for 3min.

        6. Digestion: Enzymic digestion at 37oC for 10-40min. Enzyme working buffer and Protease solution (10x) in 9:1 proportion.

        7. Washing: At room temperature, wash with cleaning solution twice, 5min each time.

        8. Dehydration: With 70%, 85% and 100% gradient EtOH for 2min.

        9. Dry at room temperature.

         

        B. Cell - Slides pretreatment

        1. Slides heating: Heat at 56oC for 30min.

        2. Washing: At room temperature, wash with cleaning solution twice, 5min each time.

        3. Dehydration: With 70%, 85% and 100% gradient EtOH for 2min.

        4. Dry at room temperature.

 

        3. DENATURATION & HYBRIDIZATION

        Denaturation

        Before probe use, invert the tube up and down for 5 times. Centrifuge instantaneously for 1-2 sec., if there is a persistent red substance, invert until the substance disappearance.

         

        A. Tissue - Slides FISH hybridization

        a). 85oC denaturation for 5min.

        b). 42oC hybridization for 2h (for pretreatment made with HealthCare Biotech FISH Pretreatment Reagent Kit) or Overnight (for pretreatment made without HealthCare Biotech FISH Pretreatment Kit Reagent).

         

        B. Cells - FISH hybridization

        a). 88oC denaturation for 2min.

        b). 45oC hybridization for 2h.

         

        4. WASHING

        1. Wash at room temperature with 2xSSC solution for 1min.

        2. Washing solution 68oC (Neutral 0.3% NP-40/0.4 x SSC solution) for 3min.

        3. Deionized water at 37oC for 1min.

        4. Dry at room temperature.

         

        5. DYEING

        DAPI counterstaining for 10 min.

         

        6. RESULTS READING

        Target area analysis under fluorescence microscope (HealthCare Biotech FastProbe® filter parameters: Green (495/518), Orange (553/565), DAPI (367/452).