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Ready For use FISH Probe Kits

Ready For use FISH Probe Kits 

By Fluorescence In Situ Hybridization Method (FISH) 


Test Item

Cat#

Product Name

Probe Name

Size

 







Non-Small Cell Lung Cancer



KL-LU001



Human ALK gene fusion 

detection probe



ALK

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T)



KL-LU002



6q probe (6q22)



ROS1

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-LU003



C-MET gene detection probe



C-MET/CEP7

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



Breast Cancer



KL-BR001



Human HER-2 gene amplification detection probe



HER2/CEP17

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T)



Stomach Cancer



KL-ST001



Human HER-2 gene amplification detection probe



HER2

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 

 







Bladder Cancer


KL-BL001 





 Bladder Cancer cells chromosome and gene anomaly detection probe


CEP3/CEP7

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-BL002



P16/CEP17

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-BL003



P53 gene probe



P53/CEP17

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



Cervical Cancer



KL-CE001



TERC gene amplification 

detection probe



TER/CEP3

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 





Brain Tumor



KL-BT001



BRAF gene probe (KIAA1549/BRAF)



BRAF

100μL (10T) + Pretreatment reagent kit (10T) + DAPI   



KL-BT003



1p/19q chromosome deletion probe



1p/19q

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T)

 










  






 

Lymphoma



KL-LY001



BCL6 gene break apart rearrangement probe



BCL6

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-LY002



BCL2 gene break apart rearrangement probe



BCL2

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-LY003



IGH gene break apart rearrangement probe



IGH

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-LY004



MYC/IGH t (8;14) gene fusion probe



IGH/C-MYC

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-LY005



BCL2/IGH t (14;18) gene fusion probe



IGH/BCL2

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-LY006



P53 gene probe



P53/CEP17

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-LY007



MYC gene break apart rearrangement probe



MYC

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-LY008



CCND1 (BCL1)/IGH gene fusion probe



CCND1/IGH

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T)  












  

 

Chronic Lymphocytic Leukemia (CLL)



KL-CLL01

 






CLL chromosome and gene anomaly detection probe



RB1/ATM

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 


KL-CLL02


P53/CEP17

100μL (10T) + Pretreatment reagent kit + DAPI (10T) 



KL-CLL03



D13S319/CEP12

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-CLL04



Chromosome 12 centromeric probe



CEP12

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-CLL05



ATM gene deletion probe



CEP11/ATM

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-CLL06



13(13q14)/13q34 probe



D13S319/LAMP1 (13q34)

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-CLL07



MYB (6q23) gene probe



MYB/CEP6

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 

 










Acute Myeloid Leukemia /AML (Non APL)



KL-AML01



RUNX1/RUNX1T1 t (8;21) gene fusion probe



AML1/ETO

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-AML02



PML/RARA gene fusion probe



PML/RARA

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-AML03



CBFB gene break apart rearrangement detection probe



CBFB

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-AML04



KMT2A (MLL) gene break apart rearrangement detection probe



MLL

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-AML05



CBFB/MYH11 gene fusion probe



CBFB/MYH11

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



Chronic Myeloid Leukemia

(CML)

 



KL-CML01

 



BCR/ABL gene fusion detection probe


  


BCR/ABL

 

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 

 















  



 

Acute Lymphoblastic Leukemia (ALL)



KL-ALL01



ETV6 (TEL)/RUNX1 (AML1) gene translocation probe



TEL/AML1

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-ALL02



BCR/ABL gene fusion detection probe



BCR/ABL

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-ALL03



IGH gene break apart rearrangement probe



IGH

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-ALL04



P16 gene deletion probe



P16 (CDK2NA)/CEP9

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-ALL05



KMT2A (MLL) gene break apart rearrangement probe



MLL

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-ALL06



Chromosomes 4, 10 probe



CEP4/CEP10

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-ALL07



Chromosome 17 centromeric probe



CEP17

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-ALL08



MYC gene break apart rearrangement probe



MYC

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-ALL09



TCF3/PBX1gene fusion probe



TCF3/PBX1

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 

 












  

Multiple Myeloma

(MM)



KL-MM001



RB1 gene deletion probe



RB1

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-MM002



1q21 gene probe



1q21

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-MM003



P53 gene probe



P53/CEP17

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-MM004



IGH gene break apart probe



IGH

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-MM005



CCND1 (BCL1)/IGH gene fusion probe



CCND1/IGH

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-MM006



13(13q14)/13q34 probe



D13S319/LAMP1(13q34)

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 

 















Myelodysplastic Syndrome

(MDS)

 

 

 



KL-MDS01

 










  

MDS chromosome and gene anomaly detection probe

 

 



D7S486/CEP7

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 


KL-MDS02


D7S522/CEP7

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 


KL-MDS03


CSF1R/D5S630

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-MDS04


EGR1/D5S630 5P15(D5S630)/5q31.2(EGR1)

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-MDS05



D20S108/CEP8

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-MDS06



CEPY/CEPX

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-MDS07



Chromosome 8 centromeric probe



CEP8

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 

  









Aplastic Anemia 



KL-AN01



Chromosome 8 centromeric probe



CEP8

100μL (10T) + Pretreatment reagent kit (10T) + DAPI(10T)  



KL-AN02



7q chromosome deletion [del(7q)] probe 



D7S486/CEP7

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-AN03



5q chromosome deletion [del(5q)] probe


EGR1/D5S630 5P15(D5S630)/5q31.2(EGR1)

100μL (10T) + Pretreatment reagent kit (10T) + DAPI (10T) 



KL-AN04



20q chromosome deletion [del(20q)] probe



20q12(D20S108)/20q13.12

100μL (10T) + Pretreatment reagent kit (10T) + DAPI 



Flexible Dye Selection

Fluorescein color

Excitation wavelength

Emission wavelength

Filter

Orange (RHO)

553

565

Orange Monochannel ; Green/Orange Dual Channel

Green (FITC)

495

518

Green Monochannel ; Green/Orange Dual Channel

DAPI (Blue)

367

452

Monochannel




Our Advantages:

a). FISH probes (FastProbe®) hybridization time of 2 hours (blood cell and tissue) against 16-24 hours for conventional probes in the market. 

b). FISH probes (FastProbe®) using the Non-Repetitive Sequences technology ensuing a product with a very good hybridization result, good and clear signal to background noise, high specificity and accuracy.

c). FISH probes (FastProbe®) with a very good anti-quenching effect allowing long observation under fluorescence microscope.

d). An excellent customer service with very short lead time from PO confirmation to shipment.

e). Very competitive affordable price. 



Fast Fluorescence In-Situ Hybridization (FISH) Probe

Protocol Manual

        1. SAMPLE

        A. Tissue

        (1) a). Paraffin-embedded tissue samples fixation with 4% formaldehyde for 6-72 hours.

           b). Thickness: 3-5µm

        (2) Glass slides: Adhesive slides.

         

        B. Cells

        (1) a). Anticoagulated fresh bone marrow (Storage conditions 4oC, within 24h).

           b). Bone marrow cells accurate fixation (Storage conditions -20oC, within 6 months).

        (2) Glass slides: Ordinary/Common glass slides.

         

        2. SAMPLE PRETREATMENT 

        Pretreatment procedure

        A. Tissue - Slides pretreatment (sample releasing agent)

        1. Slides heating: Heat at 80oC for 30min or 65oC for 2h or overnight.

        2. Dewaxing: Deparaffinization agent 68oC for 15min.

        3. Washing: Wash with anhydrous EtOH at room temperature for 5min.

        4. Permeation: Permeation agent 90oC for 20min or deionized water 90oC for 40min.

        5. Washing: Wash with distilled water at 37oC for 3min.

        6. Digestion: Enzymic digestion at 37oC for 10-40min. Enzyme working buffer and Protease solution (10x) in 9:1 proportion.

        7. Washing: At room temperature, wash with cleaning solution twice, 5min each time.

        8. Dehydration: With 70%, 85% and 100% gradient EtOH for 2min.

        9. Dry at room temperature.

         

        B. Cell - Slides pretreatment

        1. Slides heating: Heat at 56oC for 30min.

        2. Washing: At room temperature, wash with cleaning solution twice, 5min each time.

        3. Dehydration: With 70%, 85% and 100% gradient EtOH for 2min.

        4. Dry at room temperature.

 

        3. DENATURATION & HYBRIDIZATION

        Denaturation

        Before probe use, invert the tube up and down for 5 times. Centrifuge instantaneously for 1-2 sec., if there is a persistent red substance, invert until the substance disappearance.

         

        A. Tissue - Slides FISH hybridization

        a). 85oC denaturation for 5min.

        b). 42oC hybridization for 2h (for pretreatment made with HealthCare Biotech FISH Pretreatment Reagent Kit) or Overnight (for pretreatment made without HealthCare Biotech FISH Pretreatment Kit Reagent).

         

        B. Cells - FISH hybridization

        a). 88oC denaturation for 2min.

        b). 45oC hybridization for 2h.

         

        4. WASHING

        1. Wash at room temperature with 2xSSC solution for 1min.

        2. Washing solution 68oC (Neutral 0.3% NP-40/0.4 x SSC solution) for 3min.

        3. Deionized water at 37oC for 1min.

        4. Dry at room temperature.

         

        5. DYEING

        DAPI counterstaining for 10 min.

         

        6. RESULTS READING

        Target area analysis under fluorescence microscope (HealthCare Biotech FastProbe® filter parameters: Green (495/518), Orange (553/565), DAPI (367/452).